Journal: Oncology Reports

Article Title: Girdin regulates both migration and angiogenesis in pancreatic cancer cell lines

doi: 10.3892/or.2023.8606

Figure Lengend Snippet: Expression and knockdown of Girdin in PaCa cell line. (A) Expression of Girdin mRNA in normal pancreatic and PaCa cell lines. The reverse transcription-quantitative PCR experiments were calculated using a comparative quantitative method with calibration curves. The vertical axis represents the ratio of Girdin expression to that in the human pancreatic ductal epithelial cell lines. (B) Western blotting was performed using Girdin and GAPDH antibodies. The density of bands detected was measured, and the ratio of Girdin to GAPDH was plotted. (C) Transfection of PaCa cell lines with negative control siRNA or Girdin siRNA. Girdin was knocked down by transfection of Girdin siRNA for MIA PaCa-2, AsPC-1 and PANC-1. (D) The knockdown of Girdin in these cell lines was confirmed by western blotting. The data are presented as the mean ± SEM. All experiments were performed in triplicate and repeated three times. *P<0.01 [A: One-way ANOVA with Dunnett's multiple comparison test. B: Student's t-test (unpaired), two-tailed]. PaCa, pancreatic cancer; siRNA, small interfering RNA.

Article Snippet: Human PaCa cell lines [MIA PaCa-2 (cat. no. CRL-1420TM), SW 1990 (cat. no. CRL-2172TM), AsPC-1 (cat. no. CRL-1682TM), BxPC-3 (cat. no. CRL-1687TM), PANC-1 (cat. no. CRL-1469TM), Capan-2 (cat. no. HTB-80TM) ( )] and the human pancreatic duct epithelial cell line H6c7 (cat. no. PCS-600-010) were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Knockdown, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Negative Control, Comparison, Two Tailed Test, Small Interfering RNA

Journal: International Journal of Molecular Sciences

Article Title: microRNA-21 Regulates Stemness in Pancreatic Ductal Adenocarcinoma Cells

doi: 10.3390/ijms23031275

Figure Lengend Snippet: RT-qPCR analysis of miRs levels miR-21, miR-221, miR-155, and miR-126 in PDAC cell lines (BxPC3, MiaPaCa-2, Panc-1, compared with HPDE). ( A ) miR-21-5p relative expression was significantly increased in MiaPaCa-2 and Panc-1 but not in BxPC3 PDAC cell lines. ( B ) miR-155-5p relative expression was significantly overexpressed in BxPC3, MiaPaCa-2, and Panc-1 PDAC cell lines. ( C ) miR-221-5p relative expression was significantly upregulated in MiaPaCa-2 and Panc-1 but not in BxPC3. The column graphic represents the average of three replicates of RNA isolated from each cell line. ( D ) miR-126-5p relative expression was significantly reduced in PDAC cell lines. The column graphs represent the average of three replicates of RNA isolated from each cell line. Data normalized according to RNU6 expression by fold analysis ( n = 3, p < 0.05 for all). p -values are indicated as: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001; error bars indicate standard deviation (SD).

Article Snippet: Panc-1 (ATCC ® CRL-1469TM), MiaPaCa-2 (ATCC ® CRL-x1420TM), BxPC-3 (ATCC ® CRL-1687TM), and non-tumorigenic human pancreatic ductal epithelial cell line (HPDE; H6c7, ATCC ® CRL-4023) cell lines were cultured according to ATCC’s recommendations, to 80% confluence in 75 cm 2 flasks in complete Dulbecco’s Modified Eagle’s Medium (DMEM), with 10% fetal bovine serum (FBS) at 37 °C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Expressing, Isolation, Standard Deviation